normal oral epithelial cell line noe  (Celprogen Inc)

Journal: Molecular Therapy. Nucleic Acids

Article Title: MiR-449a antagonizes EMT through IL-6-mediated trans -signaling in laryngeal squamous cancer

doi: 10.1016/j.omtn.2024.102140

Figure Lengend Snippet: Expression profile of miR-449a in LSCC cell lines (A) Relative basal miR-449a expression levels (mean ± SD) in FaDu, UPCI:SCC152,HNO-210 cell lines vs. human hypopharyngeal normal cell. (B) miR-449a relative expression levels in HNO-210 cells. (C) miR-449a relative expression levels in FaDu cells. Each data point was obtained in triplicate and data are shown as mean ± SD (Student’s t test) ∗p < 0.05, ∗∗∗p < 0.001.

Article Snippet: The HNO-210 cell line was grown in DMEM (MICROGEM DMEM Cat# AL066A), while the Human hypopharyngeal normal primary cell culture (CELPROGEN, Cat# 36074-04) cells were grown in human hypopharyngeal normal primary cell culture media (CELPROGEN, Cat# M36074-04).

Techniques: Expressing

Journal: Bioengineered

Article Title: Actinidia chinensis Planch Root extract suppresses the growth and metastasis of hypopharyngeal carcinoma by inhibiting E2F Transcription Factor 1-mediated MNX1 antisense RNA 1.

doi: 10.1080/21655979.2022.2037226

Figure Lengend Snippet: Figure 1. AcRoots suppressed cell proliferation of hypopharyngeal carcinoma. (a) Incubation with acRoots lower than 200 μg/mL did not affect the cell viability of HHPCs, while acRoots more than 400 μg/mL reduced the cell viability of HHPCs. (b) Incubation with acRoots reduced cell viability of FaDu in a dosage-dependent way. (c) Incubation with acRoots reduced cell proliferation of FaDu. (d) Incubation with acRoots promoted cell apoptosis of FaDu. *, **, *** vs. 0 μg/mL Actinidia chinensis Planch Root extract, p < 0.05, p < 0.01, p < 0.001.

Article Snippet: Human hypopharyngeal primary cells (HHPCs) were purchased from Celprogen Inc. (Carlsbad, CA, USA) and cultured in Human Hypopharyngeal Normal Cell Culture Media with Serum (Celprogen) according to previous study [16].

Techniques: Incubation

Journal: Bioengineered

Article Title: Actinidia chinensis Planch Root extract suppresses the growth and metastasis of hypopharyngeal carcinoma by inhibiting E2F Transcription Factor 1-mediated MNX1 antisense RNA 1.

doi: 10.1080/21655979.2022.2037226

Figure Lengend Snippet: Figure 2. AcRoots suppressed cell migration invasion of hypopharyngeal carcinoma. (a) Incubation with acRoots reduced cell migration of FaDu. (b) Incubation with acRoots reduced the number of migrating cells in FaDu. (c) Incubation with acRoots reduced cell invasion of FaDu. *, **, *** vs. 0 μg/mL Actinidia chinensis Planch Root extract, p < 0.05, p < 0.01, p < 0.001.

Article Snippet: Human hypopharyngeal primary cells (HHPCs) were purchased from Celprogen Inc. (Carlsbad, CA, USA) and cultured in Human Hypopharyngeal Normal Cell Culture Media with Serum (Celprogen) according to previous study [16].

Techniques: Migration, Incubation

Journal: Bioengineered

Article Title: Actinidia chinensis Planch Root extract suppresses the growth and metastasis of hypopharyngeal carcinoma by inhibiting E2F Transcription Factor 1-mediated MNX1 antisense RNA 1.

doi: 10.1080/21655979.2022.2037226

Figure Lengend Snippet: Figure 3. AcRoots reduced expression of E2F1 and MNX1-AS1. (a) Expressions of E2F1 and MNX1-AS1 were up-regulated in hypopharyngeal carcinoma tissues. (b) Protein expression of E2F1 was up-regulated in hypopharyngeal carcinoma tissues. (c) Incubation with acRoots decreased expression of E2F1 and MNX1-AS1 in FaDu. (d) Incubation with acRoots decreased protein expression of E2F1 in FaDu. **, *** vs. 0 μg/mL acRoots or normal tissues, p < 0.01, p < 0.001.

Article Snippet: Human hypopharyngeal primary cells (HHPCs) were purchased from Celprogen Inc. (Carlsbad, CA, USA) and cultured in Human Hypopharyngeal Normal Cell Culture Media with Serum (Celprogen) according to previous study [16].

Techniques: Expressing, Incubation

Journal: Bioengineered

Article Title: Actinidia chinensis Planch Root extract suppresses the growth and metastasis of hypopharyngeal carcinoma by inhibiting E2F Transcription Factor 1-mediated MNX1 antisense RNA 1.

doi: 10.1080/21655979.2022.2037226

Figure Lengend Snippet: Figure 4. AcRoots reduced expression of MNX1-AS1 through inhibition of E2F1. (a) The expression of E2F1 was positively associated with MNX1-AS1 in hypopharyngeal carcinoma. (b) Transfection with pcDNA- E2F1 promoted mRNA expression of E2F1 in FaDu. (c) Transfection with pcDNA-E2F1 promoted expression of MNX1-AS1 in FaDu. (d) Transfection with pcDNA-E2F1 promoted protein expression of E2F1 in FaDu. (e) Overexpression of E2F1 attenuated Actinidia chinensis Planch Root extract-induced decrease of E2F1 mRNA. (f) Overexpression of E2F1 attenuated Actinidia chinensis Planch Root extract-induced decrease of MNX1-AS1. (g) Overexpression of E2F1 attenuated Actinidia chinensis Planch Root extract-induced decrease of E2F1 protein. **, *** vs. NC, p < 0.01, p < 0.001. ## vs. acRoots, p < 0.01.

Article Snippet: Human hypopharyngeal primary cells (HHPCs) were purchased from Celprogen Inc. (Carlsbad, CA, USA) and cultured in Human Hypopharyngeal Normal Cell Culture Media with Serum (Celprogen) according to previous study [16].

Techniques: Expressing, Inhibition, Transfection, Over Expression

Journal: Bioengineered

Article Title: Actinidia chinensis Planch Root extract suppresses the growth and metastasis of hypopharyngeal carcinoma by inhibiting E2F Transcription Factor 1-mediated MNX1 antisense RNA 1.

doi: 10.1080/21655979.2022.2037226

Figure Lengend Snippet: Figure 5. AcRoots suppressed cell proliferation and metastasis of hypopharyngeal carcinoma through regulation of E2F1. (a) Overexpression of E2F1 attenuated acRoots-induced decrease in cell viability in FaDu. (b) Overexpression of E2F1 attenuated acRoots-induced increase in cell apoptosis in FaDu. (c) The relative apoptosis rate in acRoots-induced FaDu with or without pcDNA- E2F1 transfection. (d) Overexpression of E2F1 attenuated acRoots-induced decrease in cell migration in FaDu. (e) Overexpression of E2F1 attenuated acRoots-induced decrease in cell invasion in FaDu. **, *** vs. NC, p < 0.01, p < 0.001. ### vs. acRoots, p < 0.001.

Article Snippet: Human hypopharyngeal primary cells (HHPCs) were purchased from Celprogen Inc. (Carlsbad, CA, USA) and cultured in Human Hypopharyngeal Normal Cell Culture Media with Serum (Celprogen) according to previous study [16].

Techniques: Over Expression, Transfection, Migration

Journal: Bioengineered

Article Title: Actinidia chinensis Planch Root extract suppresses the growth and metastasis of hypopharyngeal carcinoma by inhibiting E2F Transcription Factor 1-mediated MNX1 antisense RNA 1.

doi: 10.1080/21655979.2022.2037226

Figure Lengend Snippet: Figure 6. AcRoots suppressed in vivo hypopharyngeal carcinoma growth. (a) Intragastrically administered acRoots repressed tumor growth of hypopharyngeal carcinoma with reduced tumor volume and weight. (b) No sudden weight loss of mice was observed in the acRoots group, and there was no significant difference in weight between different groups. (c) Intragastrically administered acRoots reduced the expression of E2F1 and MNX1-AS1. (d) Immunohistochemical analysis indicated down-regulation of E2F1 and Ki67 in the tumor tissues. Incubation with acRoots increased the number of TUNEL-positive cells in the tumor tissues. *, *** vs. 0 mg/mL acRoots, p < 0.05, p < 0.001.

Article Snippet: Human hypopharyngeal primary cells (HHPCs) were purchased from Celprogen Inc. (Carlsbad, CA, USA) and cultured in Human Hypopharyngeal Normal Cell Culture Media with Serum (Celprogen) according to previous study [16].

Techniques: In Vivo, Expressing, Immunohistochemical staining, Incubation, TUNEL Assay